Phospholipid oxidation and carotenoid supplementation in Alzheimer’s disease patients


Creators: Ademowo, Stella, Dias, Irundika, Milic, Ivana, Devitt, Andrew, Moran, Rachel, Mulcahy, Riona, Howard, Alan, Nolan, John and Griffiths, Helen
Keywords: oxidative stress, lipid peroxidation, POVPC, mass spectrometry, lutein, eso-zeaxanthin, zeaxanthin, cognitive function, supplementation
Divisions: College of Health and Life Sciences > Cell and Tissue Biomedical Research
Funders: Biotechnology and Biological Sciences Research Council, Kidney Research Foundation, ARUK, The Howard Foundation, Aston Research Centre for Healthy Ageing
Data Publisher: Aston University
Date Made Available: 16 March 2017
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Data Collection and Processing Methods: Data was obtained from Alzheimer’s disease (AD) patients (attending the Age-Related Care Unit at Waterford University Hospital Ireland and healthy control subjects with the same age) prior to the administration of carotenoid (antioxidant) supplements and six months after commencement of supplementation. Mild to moderate AD was defined as an average Mini-Mental State Examination (MMSE) score of 14-24, with some alteration in behaviour as well as difficulties in carrying out day-to-day tasks. Cognitive function was assessed with different cognition tests which includes a semantic fluency score using ‘Animal’ category (as many examples in one minute), and a phonemic fluency score using the ‘FAS’ test (as many words starting with letters F, A and S, one minute per letter) The novel oxidised phospholipid biomarker 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) was analysed using electrospray ionization tandem mass spectrometry (MS) with multiple reaction monitoring (MRM), 8-isoprostane (IsoP) was measured by ELISA and ferric reducing antioxidant potential (FRAP) was measured by a colorimetric assay. All statistical analyses were performed using Prism statistical analysis software package (Version 7).
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